Renal tissue remodeling. A. Representative renal trichrome (×20), dihydroethidium (DHE) (×20), CD31 immunofluorescence (×40), α-SMA (×20) and PAS staining (×20). Kidney fibrosis increased in hypertension (HT) and coronary artery stenosis (CAS) compared with Sham; kidney fibrosis and glomerular score increased synergistically in CAS+HT compared with the other groups (B, C). DHE staining (normalized to DAPI-positive nuclei) increased in CAS compared with Sham, and in CAS+HT compared with Sham and HT (D). Capillary density (CD31 immunofluorescence) in HT decreased compared with Sham, but in CAS +HT fell lower than all other groups (E). Renal microvascular media-to-lumen ratio (α-SMA) increased in CAS compared with Sham, and further in HT and CAS+HT compared with Sham and CAS (F). Tubular injury score (PAS staining) increased in HT and CAS, and further aggravated in CAS+HT (G). *P<0.05 vs. Sham; #P<0.05 vs. HT; &P<0.05 vs. CAS; Δ
P<0.05 for synergistic interaction CAS×HT. Scale bar = 50μm.