(A) Anti-VEGFR-3 (7μg) or control mAb (7μg) were injected into pinnae with or without PI3Kα inhibitor (PIK2, 10μg) or pan-PI3K activator (740Y-P, 50μg). Six hours later, the ear skin sheets were stained for LYVE-1, CCL21, and HS. Original magnification 200×. Bar, 100μm. (B) Same as (A), staining intensity of CCL21 and HS, and % CCL21- and HS-positive area in LYVE-1+ lymphatics were calculated. n = 10-12 fields/group. Results from 2 mice/group, 3 independent experiments. * p<0.05 by one-way ANOVA.