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. Author manuscript; available in PMC: 2016 Mar 31.
Published in final edited form as: Circulation. 2015 Feb 20;131(13):1202–1213. doi: 10.1161/CIRCULATIONAHA.114.012669

Figure 1.

Figure 1

Characterization of Adamts7−/− Mice. A) Assessment of Adamts7 expression in multiple tissues from the mouse (N=3). Data is shown as relative quantity (to β-actin) and represented as fold-change over the lowest expressing tissue. B) Schematic of the targeting strategy used in creation of the Adamts7−/− mouse, and the location of TaqMan probesets used in confirmation of KO of the gene expression. C) TaqMan realtime qPCR measurements of expression of Adamts7 in heart and lung tissue from WT and KO mice (N=6/group) showing near complete loss of expression of Adamts7. Data from the two probe-sets identified in Figure 1B are shown. D) Overnight X-gal staining of select tissues from WT and KO animals confirms the realtime qPCR data and further illustrates expression patterns of Adamts7 in these tissues.