Table 2.

Primers and annealing temperature employed in the analysis of the TP53 gene

	Primers	Annealing T (°C)
Exon 4 forward	GCTCTTTTCACCCATCTACA	53
Exon 4 reverse	ACCGTGCAAGTCACAGACTT
Exon 5 forward	CTCTTCCTACAGTACTCCCCTGCC	62
Exon 5 reverse	GCCCCAGCTGCTCACCATCGCTA
Exon 6 forward	GATTGCTCTTAGGTCTGGCCCCT
Exon 6 reverse	GGCCACTGACAACCACCCTTAAC
Exon 7 forward	GTGGTTATCTCCTAGGTTGGCTCT
Exon 7 reverse	CAAGTGGCTCCTGACCTGGAGTC
Exon 8 forward	AGTAGTGGTAATCTACTGGGACGG	54
Exon 8 reverse	GCAGCTCGTGGTGAGGCT
Exon 9 forward	CCCTTCAGGTACTAAGTCTTGG	55
Exon 9 reverse	CGAAATGCCCCAATTGCAGG