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. 2015 Feb 23;37(2):120–126. doi: 10.1016/j.bjhh.2015.02.007

Figure 4.

Figure 4

Chromatographic profile of a heterozygous trait for Hb D-Punjab (B and C) and a double heterozygous Hb S/D-Los Angeles A and D). (A and B) The VARIANT I system chromatogram with heterozygous beta-thalassemia analysis kit. The arrow indicates the pike corresponding to Hb D-Los Angeles with average retention time of 4.1–4.3 min. It is possible to note decreases in Hb A2 values, a characteristic of samples with this hemoglobin variant. (C and D) The ultra2 Resolution system chromatogram with Genesys analysis kit. The arrow indicates the Hb D-Punjab spike, with retention time between 0.92 and 0.96 min related to Hb S.