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Agarose gel visualization of fragments generated by the PCR-RFLP technique for screening of β121 Glu→Gln (GAA→CAA) mutation. Normal alleles generate two fragments and mutant alleles maintain the 564 bp fragment, indicated by the arrows. N: absent mutation; HET: heterozygous for Hb D-Los Angeles; mm: molecular marker of 100 bp. Restriction enzyme: EcoRI (G↓AATTC).
