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. 2015 Mar 31;4:10.3402/jev.v4.25530. doi: 10.3402/jev.v4.25530

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© 2015 Vendula Pospichalova et al.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License, permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2 — Isolation of microvesicles (MVs) and exosomes (exs) by ultracentrifugation (UC) and sucrose cushion. A schematic representation of protocol used to isolate extracellular vesicles (EVs). Black boxes describe the purpose of each centrifugation step, blue boxes contain additional instructions/explanation. Optional filtration step is indicated by italics. Green boxes depict fraction after centrifugation at relative centrifugation force (RCF) indicated by number (in thousands×g). P, pellet; S, supernatant.