Table 1. Determination of plitidepsin IC50 in human and murine JAK2V617F-mutated cell lines and controls.
| Cell line |
IC50
(nm) |
|
|---|---|---|
| WST-1 assay | Clonogenic assay | |
| K562 | 1.50±0.10 | 2.70±0.30 |
| HEL | 1.00±0.30 | 1.50±0.05 |
| SET2 | 1.00±0.30 | 0.80±0.02** |
| UKE-1 | 2.40±0.20 | 0.50±0.03** |
| Ba/F3 JAK2 wild-type | 0.40±0.03 | ND |
| Ba/F3 JAK2 V167F | 0.03±0.01** | ND |
Abbreviations: IC50, plitidepsin concentration that reduced colony number to 50% that measured in control dishes with vehicle only; ND, not done.
IC50 value was calculated using both short-term proliferation assay in liquid cultures and long-term clonogenic assay in agar. Control murine Ba/F3 wild-type cells were maintained in the presence of IL-3. **P<0.01.