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. 2015 Mar 13;6:6463. doi: 10.1038/ncomms7463

Figure 2. Detection of β-galactosidase activities in living ovarian cancer cells with HMRef-βGal.

Figure 2

(a) Activation of HMRef-βGal on enzymatic reaction with β-galactosidase. Photographs show cuvettes containing the reaction mixture irradiated with 365-nm ultraviolet light. (b) Time course of enzymatic reaction of HMRef-βGal with β-galactosidase. β-Galactosidase (5 units) was added at 50 s. HMDER-βGal was used as a control. Probe concentrations were 0.5 μM. (c) Confocal images of ovarian tumour cells treated with HMRef-βGal. Cells were incubated with 10 μM HMRef-βGal for 1 h, and DIC and fluorescence images were obtained. Ex/Em=498 nm/505–600 nm. Scale bar, 100 μm. (d) Fluorescence intensity of ovarian cancer cells treated with HMRef-βGal in the presence or absence of β-GA. Fluorescence intensity of cells on a 96-well plate was measured with a plate reader (Ex/Em=498 nm/518 nm). Probe concentration was 10 μM. Data represent means±s.e.m (n=4).