Figure 2.

Expression of cytosolic GCaMP3 and Lck-GCaMP3 throughout astrocytes. (A,B) Cartoons of differences between cytosolic and membrane-targeted GECIs. (C) Schematic illustrates the protocol for AAV 2/5 microinjections into the hippocampus. The right-hand image shows the expression of Lck-GCaMP3 throughout the hippocampus, and D shows expression within the stratum radiatum region for Lck-GCaMP3 and cyto-GCaMP3. (E) Representative images showing GFAP and GCaMP3 staining for the stratum radiatum region from control mice that received no AAVs and those that received AAV2/5 Lck-GCaMP3. The image is a zoomed-out view. AAV, adeno-associated virus; gc, genome copy; PM, plasma membrane. (From Shigetomi et al. 2013a; reproduced, with permission, from the authors as well as by the Creative Commons license for reuse under the public domain.)