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. 2015 Jan 11;6(1):1. doi: 10.1186/2049-1891-6-1

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© Mohamad-Fauzi et al.; licensee BioMed Central. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Transfection and integration efficiency in MSCs. P5 MSCs (A) and 9004 BM-MSC at passages 10, 15 and 20 (B) were transfected with pEGFP-N1. Integrant number were expressed as the number of discrete colonies that were resistant to G418 selection. Statistically significant groups by Tukey’s test is indicated by letters (P < 0.05). 9004 BM-MSC showed the highest number of integrants, followed by 9003 ASC and 9003 BM-MSC. 9004 BM-MSC showed a decreasing trend in number of integrants with increasing passages.