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. 2015 Jan 11;6(1):1. doi: 10.1186/2049-1891-6-1

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© Mohamad-Fauzi et al.; licensee BioMed Central. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Osteogenic differentiation of MSCs and fibroblasts. P5 MSCs from 9004 BM-MSC (A, B), 9003 BM-MSC (C, D), 9003 ASC (E, F) and 1014 EF (G, H) were cultured in osteogenic differentiation or control medium, respectively and stained with Alizarin Red S, which stains calcium deposits red. Calcium mineralization in 9003 ASC (E) appeared less extensive than that of the BM-MSCs (A and C). 1014 EF failed to undergo osteogenesis, as evident from the absence of staining. (G, H;). Representative images are shown at 200X magnification. Scale bars represent 100 μm.