FIG. 1.

Schematic showing hepatocyte and liver sinusoidal endothelial cell (LSEC) coculture configurations. Hepatocytes and LSECs are cocultured as stacked layers in sandwich collagen gel. Coculture configurations with collagen–hepatocyte–collagen–LSEC–collagen (C1), collagen–LSEC–collagen–hepatocyte–collagen (C2), and collagen–(hepatocyte+LSEC)–collagen (C3) were prepared and single cell controls with collagen–hepatocyte–collagen with 50% hepatocyte coverage (C4) and collagen–LSEC–collagen (C5) were prepared.