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. 2015 Mar 18;17(1):64. doi: 10.1186/s13075-015-0587-4

Figure 4.

Figure 4

Neutrophil respiratory burst and granulation induced by patient-derived PR3-ANCA-positive IgG or MPO-ANCA-positive IgG in HMGB1-primed neutrophils. Neutrophil respiratory burst induced by patient-derived PR3-ANCA-positive IgG (A) or MPO-ANCA-positive IgG (B) was measured by conversion of dihydrorhodamine to rhodamine-123 in HMGB1-primed cells. (C and D) were representative histograms showing that PR3-ANCA-positive IgG and MPO-ANCA-positive IgG induced respiratory burst in HMGB1-primed neutrophils. Neutrophil granulation induced by patient-derived PR3-ANCA-positive IgG (E) or MPO-ANCA-positive IgG (F) was measured. Bars represent mean ± standard deviation (SD) of repeated measurements on neutrophils of 8 independent experiments and donors. All statistical differences between groups were shown in the Table S1 in Additional file 3. ANCA, antineutrophil cytoplasmic antibody; HMGB1, high mobility group box-1; IgG, immunoglobulin G; MPO, myeloperoxidase; PR3, proteinase 3.