Fig. 3.

Expression of complement component C3 gene at different developmental stages. (A) Larvae was harvested during post fertilization to 28 days after hatching. The RNA extracted and analyzed by RT-PCR method. (B) The mRNA expression of C3 was analyzed by real-time PCR. Different letters over each bar with the standard deviation represent significant differences one group according to unpaired matched comparisons (*P<0.05). Different four stages of embryo (~0.92 mm) including fertilized egg (F), molura (E1), blastula (E2), late neurula (E3) and ten larval stages (~11.34 mm) including 0 to 28 days post hatching (dph) (H0, H1, H2, H3, H4, H5, H7, H14, H21 and H28).