Figure 1.

Effects of 5-fluorouracil (5-FU), 5-FU in combination with levofolene (LF) and doxorubicin (DOXO) on growth inhibition of HaCaT cell line. The cells were seeded and treated with different concentrations of 5-FU (A) or 5-FU+LF (B) or DOXO (C) for 24, 48 and 72 h and thereafter cell growth was evaluated by MTT assay as described in Materials and methods. The effects on cell growth were expressed as % of control. The experiments were performed three times. The bars represent means ± SD of three independent experiments.