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. 2015 Mar 17;46(5):2067–2075. doi: 10.3892/ijo.2015.2930

Figure 1.

Figure 1

Differential gene expression in confluent (90%) and subconfluent (50%) MDA-MB-231 breast cancer cells. (A) Hierarchical clustering analysis of confluent and subconfluent MDA-MB-231 breast cancer cells. Genes with an expression level exceeding a 2-fold difference relative to the median value were selected for hierarchical clustering analysis (n=2,234 genes). The data are presented in matrix format in which rows represent individual genes and columns represent each culture condition. Each cell in the matrix represents the expression level of a gene feature in an individual culture. The color red or green in cells reflects relative high or low expression levels, respectively, as indicated in the scale bar (log2-transformed scale). (B) Real-time reverse transcription polymerase chain reaction (RT-PCR) validation of interleukin-8 (IL-8) and S100A4 expression using RT-PCR and (C) confirmation of IL-8 and S100A4 protein with western blot analysis. Values shown are means ± standard deviation (SD) from three independent experiments. **P<0.01.