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. 2015 Mar 17;46(5):2067–2075. doi: 10.3892/ijo.2015.2930

Figure 2.

Figure 2

Differential gene expression between MDA-MB-231 breast cancer cells cultured in minimum essential medium (MEM) supplemented with 10 and 0.1% fetal bovine serum (FBS). (A) Hierarchical clustering analysis of cells maintained in MEM containing 10% FBS and MEM containing 0.1% FBS. Genes with an expression level exceeding a 2-fold difference relative to the median value were selected for hierarchical clustering analysis (n=2,981 genes). The data are presented in matrix format as described in Fig. 1. (B) Real-time reverse transcription polymerase chain reaction (RT-PCR) analysis of interleukin-8 (IL-8) and S100A4 expression. (C) Western blot analysis of IL-8 and S100A4. Values shown are means ± standard deviation (SD) from three independent experiments. **P<0.01.