Figure 3.

Differential gene expression between MDA-MB-231 breast cancer cells cultured in minimum essential medium (MEM) supplemented with 10% fetal bovine serum (FBS) and cells cultured in MEM containing 10% horse serum (HS). (A) Hierarchical clustering analysis of cells maintained in 10% FBS and 10% HS. Genes with an expression level exceeding a 2-fold difference relative to the median value were selected for hierarchical clustering analysis (n=2,981 genes). (B) Real-time reverse transcription polymerase chain reaction (RT-PCR) analysis of interleukin-8 (IL-8) and S100A4. (C) Western blot analysis of IL-8 and S100A4. Values shown are means ± standard deviation (SD) from three independent experiments. ^**P<0.01.