Figure 4.

Differential gene expression between MDA-MB-231 breast cancer cells maintained in different media formulations. (A) Hierarchical clustering analysis of MDA-MB-231 breast cancer cells cultured in minimum essential medium (MEM), Dulbecco’s modified Eagle’s medium (DMEM), and Roswell Park Memorial Institute (RPMI)-1640 medium that were all supplemented with 10% fetal bovine serum (FBS). (B) Real-time reverse transcription polymerase chain reaction (RT-PCR) analysis of interleukin-8 (IL-8) and S100A4. (C) Protein levels of IL-8 and S100A4 were measured by western blot analysis. Values shown are means ± standard deviation (SD) from three independent experiments. ^**P<0.01.