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. 2015 Mar 17;46(5):2067–2075. doi: 10.3892/ijo.2015.2930

Figure 5.

Figure 5

Expression of epithelial-mesenchymal transition (EMT) markers in MDA-MB-231 breast cancer cells cultured in minimum essential medium (MEM) and Dulbecco’s modified Eagle’s medium (DMEM) formulations. (A) MDA-MB-231 breast cancer cells were cultured in MEM or DMEM containing 10% fetal bovine serum (FBS) for 48 h. Real-time reverse transcription polymerase chain reaction (RT-PCR) analysis of vimentin (VIM), CDH1, CD44, and CD24 gene expression. To determine if changes in gene expression were reversible, the culture media was changed from MEM to DMEM (or from DMEM to MEM) and the cells were analyzed 48 h later. Fold-increase refers to the ratio of mRNA levels relative to that of cells cultured in MEM. (B) Western blot analysis comparing protein expression of VIM, E-cadherin, CD44, and CD24. *P<0.05, **P<0.01.