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. 2015 Mar 9;46(5):1901–1912. doi: 10.3892/ijo.2015.2923

Figure 3.

Figure 3

(A) Cloning and expression of the fused protein LASP-1-GFP. The LASP-1 CDS was cloned upstream the GFP gene in a TA-plasmid with the strong promoter CMV and containing the ampicillin and neomycin selectable marker genes. (B) The HA22T/VGH cells were transiently transfected with the plasmid pGFP-LASP1 and the cell extracts were evaluated for the endogenous LASP-1 expression of (32 kDa) and for the fused protein (59 kDa) by western blotting (WB) using anti-LASP-1 (1v=1 volume; 2v=2 volumes). (C) Expression and localization of the LASP-1-GFP fused protein in HA22T/VGH transfected cells; magnification, ×63.