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. 2015 Mar 17;46(5):1924–1934. doi: 10.3892/ijo.2015.2931

Figure 4.

Figure 4

Effects of quercetin aglycone and PAC DP-9 on expression, activation and localization of EGFR in SKOV-3 and OVCAR-8 cells. (A) Expression of total and activated EGFR on cranberry flavonoid treated SKOV-3 and OVCAR-8 cells. Cells were serum-deprived for 4 h and then treated with 5, 20 or 40 μg/ml quercetin aglycone or PAC DP-9 in serum-free DMEM medium for 12 h. Actin was probed as internal loading control. (B) Effects of PAC DP-9 on EGFR expression and localization in SKOV-3 cells. Cells were treated with different concentrations (12.5–200 μg/ml) of PAC DP-9 for 3 h before fluorescent microscopic analysis. EGFR was visualized by Alexa Fluor 594 secondary antibody (red), and nuclei were stained with DAPI.