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. 2015 Mar 17;46(5):1924–1934. doi: 10.3892/ijo.2015.2931

Figure 6.

Figure 6

Effect of PAC DP-9 and quercetin aglycone on cell cycle regulation in SKOV-3 and OVCAR-8 cells. (A) Western blot analysis of ERK-MAPK signal components and cell cycle regulatory factors in SKOV-3 and OVCAR-8 cells after treatment with quercetin aglycone or PAC DP-9 (80 μg/ml) for 0–24 h. Tubulin was probed as an internal loading control; (B) EGFR co-immunoprecipitation on SKOV-3 and OVCAR-8 cell lysates. 1, SKOV-3 untreated input control; 2, OVCAR-8 untreated input control; 3, SKOV-3 EGFR-co-immunoprecipitation; 4, OVCAR-8 EGFR-co-immunoprecipitation; 5, SKOV-3 IgG-co-immunoprecipitation control; 6, OVCAR-8 IgG-co-immunoprecipitation control. (C and D) Determination of cell cycle progression by FACS analysis in SKOV-3 and OVCAR-8 cells treated with 50 or 100 μg/ml quercetin aglycone or PAC DP-9 for 24–48 h. Data are presented as relative fluorescence intensity of G0/G1, S and G2/M phase subpopulations in 2-D charts (C) or tables (D).