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. 2015 Mar 2;46(5):1913–1923. doi: 10.3892/ijo.2015.2911

Figure 1.

Figure 1

Molecular biology and cytogenetic validation of primary colon cultures. (A) T88 and (B) T93 cell culture images in a bright field with ×20 magnification (scale bar, 50 μm). (C–E) Karyotype analysis of T93 cell cultures; (C) multicolor-FISH (M-FISH) showing a reciprocal translocation between chromosomes 20 and 22; (E) high resolution multicolor chromosome banding (MCB) image of the normal chromosome 22 compared to the rearranged chromosome 22; (D) FISH with subtelomeric probes for the p (green) and q (red) arms of chromosome 20. (F and G) Microsatellite instability (MIN) assay of T88 cell culture performed on DNA from (F) peripheral blood cells and (G) tumor tissue (TT). Black arrows indicate altered microsatellites.