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. 2015 Mar 2;46(5):1913–1923. doi: 10.3892/ijo.2015.2911

Figure 2.

Figure 2

Protein and RNA expression analysis of T88 and T93 primary cell cultures. (A–B) Real-time RT-PCR analysis of Twist1, Snail and cyclooxygenase-2 (COX2), performed on healthy mucosa (HM), tumor tissue (TT), untreated tumor cell cultures (T) from patient (A) no. 88 and (B) no. 93. (C–D) Western blot assay of E-cadherin, CD44, vimentin, Snail and actin performed with 30 μg of proteins extracted from HM, TT and untreated T from patient (C) no. 88 and (D) no. 93. (E–F) RT-PCR analysis of cytokeratin 20 and 18 performed on cDNA from HM, TT and untreated T from patient (E) no. 88 and (F) no. 93.