. Author manuscript; available in PMC: 2015 Jul 7.

Published in final edited form as: Sci Transl Med. 2015 Jan 7;7(269):269ra3. doi: 10.1126/scitranslmed.3010597

Table 3. Interaction of 4-hydroxy-2-pyridones with InhA.

Compound	Mtb MIC₅₀ (μM)	InhA IC₅₀ (μM)	Thermaflor assay (Tm °C)			ITC (+NADH)
Compound	Mtb MIC₅₀ (μM)	InhA IC₅₀ (μM)	E-NAD⁺-I	E-NADH-I	ΔTm	Kd (μM)	ΔH (cal.mol^-1)	ΔS (cal.mol-1)	N
NITD-529	1.54	9.6±0.7	50.3	54.7	4.4	25 ± 7	13100 ± 4202	-22.9	0.97 ± 0.23
NITD-564	0.16	0.59±0.05	56.3	57.8	1.5	0.56 ± 0.07	11440 ± 250	-9.8	1.04 ± 0.02
NITD-916	0.05	0.57±0.04	55	56.3	1.3	-	-	-	-
NITD-560	> 20	> 20	-	-	-	-	-	-	-
NITD-716	2.67	4.3±0.5	-	-	-	-	-	-	-
NITD-526	>20	> 20	-	-	-	-	-	-	-
PT70^a	11^a	<0.05^a	66.7	60.1	-6.6	-	-	-	-
PT166^b	10.5^b	<0.05^b	52.3	71.5	19.2	-	-	-	-

- ‘not determined’;

Pan et al 2014 (19);

Schiebel et al 2014 (18)