Figure 4. Hierarchical clustering and principal components analysis of transcriptomes.
(A) Hierarchical clustering of sorted neuron molecular profiles (top 15% probesets by coefficient of variation), showing distinct groups of transcripts enriched in IB4+SNS-Cre/TdT+, IB4−SNS-Cre/TdT+, and Parv-Cre/TdT+ neuron populations. (B) Principal component analysis shows distinct transcriptome segregation for the purified populations along three principal components axes.