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. 2015 Mar 29;16:38. doi: 10.1186/s12882-015-0034-4

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© Dai et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Angptl3 orchestrated integrin α3β1, ILK and p53, rather than caspase 3 in podocytes on treatment with PAN. (a) Western blots were performed on the expression of integrin α3, total integrin β1, phospho-integrinβ1, and ILK in podocytes with different treatment, it was showed that, there was no significant difference of in the observed molecular expression in podocytes without PAN pretreatment, while, after treatment with PAN, expression of integrin α3 and total integrin β1 became lower (ILK and phospho- integrin β1, higher) in the rm-Angptl3 group than that in the control group, and Angptl3 siRNA group showed higher expression of integrin α3, total integrin β1 (ILK, lower) than in control siRNA group, while, phospho- integrinβ1 in the two group had no statistical difference. (b, c, d, e) Relative levels of integrin α3, total integrin β1, phospho- integrinβ1 and ILK. (f, g) Western blots suggesting that there was rarely no activated caspase 3 in any observed groups; with PAN pretreatment, p53 protein level was markedly increased in rm-Angptl3 group with comparison to the control group, while, p53 protein level in Angptl3 siRNA group was a little lower than that in the control siRNA group, no p53 protein was detected in any other groups. (*P<0.05, **P<0.01, ***P<0.0001 n=6). Angptl3, angiopoietin-like3; ILK, integrin-linked kinase; PAN, puromycin aminonucleoside; siRNA, small interfering RNA.