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. Author manuscript; available in PMC: 2015 Apr 2.
Published in final edited form as: Cell Cycle. 2008 Sep 26;7(18):2916–2921. doi: 10.4161/cc.7.18.6682

Figure 1.

Figure 1

Intravital imaging of the orthotopic tumor microenvironment using the OV100 demonstrates GFP-expressing splenocyte interactions with GFP-RFP micrometastases within the pancreas. GFP-RFP tumor-bearing animals were given a single i.v. injection of GFP-expressing splenocytes. The animals were imaged at 9 days after splenocyte injection. In vivo image acquisition with the GFP longpass filter allows simultaneous signal acquisition from the GFP-RFP micrometastases adjacent to the primary tumor as well as the GFP-expressing splenocytes within the surrounding pancreatic tissue. High resolution images (A and B) can be acquired in vivo due to the short image acquisition times. Imaging was with the Olympus OV100