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. Author manuscript; available in PMC: 2015 Apr 2.
Published in final edited form as: Cell Cycle. 2008 Sep 26;7(18):2916–2921. doi: 10.4161/cc.7.18.6682

Figure 3.

Figure 3

Multicolor imaging using different fluorescence emission filters allows for discrimination between cancer cells and DsRed-expressing splenocytes within the native pancreas in vivo. GFP-RFP tumor-bearing animals were given a single i.v. injection of DsRed-expressing splenocytes. The animals were imaged at 9 days after splenocyte injection. (A) Use of the GFP longpass filter allows visualization of both green and red fluorescent signal from the cancer cells as well as the isolated red signal from the splenocytes. (B) The GFP narrow-band filter allows imaging of the GFP signal from the cancer cell nuclei. Cancer cell fragments can be discriminated from whole cells based on morphology. (C) The RFP narrow-band filter shows DsRed2 signals from the cancer-cell cytoplasm as well as DsRed expressing splenocytes. Imaging was with the Olympus OV100