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. 2015 Apr 2;3:17. doi: 10.1186/s40478-015-0191-5

Table 1.

Primary antibodies used for immunohistochemistry

Glial fibrillary acidic protein (GFAP) Monoclonal mouse Sigma-Aldrich, St. Louis, MO 1:1000
Glial fibrillary acidic protein (GFAP) Polyclonal rabbit DAKO, Carpinteria, CA 1:1000
Glial fibrillary acidic protein (GFAP) Polyclonal chicken Covance, Berkeley, CA 1:500
Vimentin Monoclonal mouse DAKO 1:500
Nestin Monoclonal mouse Covance 1:500
Glutamine synthetase Monoclonal mouse MIllipore, San Diego, CA 1:1000
Glutamine synthetase Polyclonal rabbit Santa Cruz Biotechnology, Santa Cruz, CA 1:200
EAAT1 (GLAST) Monoclonal mouse Novocastra Lab, Newcastle upon Tyne, UK 1:100
EAAT2 (GLT-1) Polyclonal guinea-pig Millipore 1:1000
EAAT2 (GLT-1) Polyclonal rabbit Cell Signaling Technology, Inc, Boston, MA 1:100
S100 Polyclonal rabbit DAKO 1:400
CD44 Monoclonal mouse DAKO 1:80
CD44 Monoclonal rat Millipore 1:200
alphaB-Crystallin Polyclonal rabbit Stressgen, Canada 1:300
SPARC/Osteonectin Polyclonal goat R&D Systems, Inc., Minnezpolis, MN 1:200
SPARC/Osteonectin Polyclonal rabbit Cell Signaling 1:200
MAP2 Monoclonal mouse Sigma-Aldrich 1:250
Pan-neuronal neurofilament marker SMI 311 Monoclonal mouse Covance 1:1000
NeuN Monoclonal mouse Millipore 1:100
CD68 Monoclonal mouse DAKO 1:300
LN-3 Monoclonal mouse ICN Biomedicals, Inc., Aurora, OH 1:50
Iba-1 Polyclonal rabbit Biocare Madical, LLC, Concord, CA 1:500
Phospho-S6 ribosomal protein (Ser 235/236)* Monoclonal rabbit Cell Signaling 1:200
Phospho-S6 ribosomal protein (Ser 240/244)* Monoclonal rabbit Cell Signaling 1:200
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Monoclonal rabbit Cell Signaling 1:100
Tumor necrosis factor alpha (TNF alpha) Polyclonal goat Santa Cruz
Neurotropin receptor p75NTR Polyclonal rabbit Promega Corp.,Madison, WI 1:500

*- these two antibodies gave similar results.