Table 1.
Glial fibrillary acidic protein (GFAP) | Monoclonal mouse | Sigma-Aldrich, St. Louis, MO | 1:1000 |
Glial fibrillary acidic protein (GFAP) | Polyclonal rabbit | DAKO, Carpinteria, CA | 1:1000 |
Glial fibrillary acidic protein (GFAP) | Polyclonal chicken | Covance, Berkeley, CA | 1:500 |
Vimentin | Monoclonal mouse | DAKO | 1:500 |
Nestin | Monoclonal mouse | Covance | 1:500 |
Glutamine synthetase | Monoclonal mouse | MIllipore, San Diego, CA | 1:1000 |
Glutamine synthetase | Polyclonal rabbit | Santa Cruz Biotechnology, Santa Cruz, CA | 1:200 |
EAAT1 (GLAST) | Monoclonal mouse | Novocastra Lab, Newcastle upon Tyne, UK | 1:100 |
EAAT2 (GLT-1) | Polyclonal guinea-pig | Millipore | 1:1000 |
EAAT2 (GLT-1) | Polyclonal rabbit | Cell Signaling Technology, Inc, Boston, MA | 1:100 |
S100 | Polyclonal rabbit | DAKO | 1:400 |
CD44 | Monoclonal mouse | DAKO | 1:80 |
CD44 | Monoclonal rat | Millipore | 1:200 |
alphaB-Crystallin | Polyclonal rabbit | Stressgen, Canada | 1:300 |
SPARC/Osteonectin | Polyclonal goat | R&D Systems, Inc., Minnezpolis, MN | 1:200 |
SPARC/Osteonectin | Polyclonal rabbit | Cell Signaling | 1:200 |
MAP2 | Monoclonal mouse | Sigma-Aldrich | 1:250 |
Pan-neuronal neurofilament marker SMI 311 | Monoclonal mouse | Covance | 1:1000 |
NeuN | Monoclonal mouse | Millipore | 1:100 |
CD68 | Monoclonal mouse | DAKO | 1:300 |
LN-3 | Monoclonal mouse | ICN Biomedicals, Inc., Aurora, OH | 1:50 |
Iba-1 | Polyclonal rabbit | Biocare Madical, LLC, Concord, CA | 1:500 |
Phospho-S6 ribosomal protein (Ser 235/236)* | Monoclonal rabbit | Cell Signaling | 1:200 |
Phospho-S6 ribosomal protein (Ser 240/244)* | Monoclonal rabbit | Cell Signaling | 1:200 |
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) | Monoclonal rabbit | Cell Signaling | 1:100 |
Tumor necrosis factor alpha (TNF alpha) | Polyclonal goat | Santa Cruz | |
Neurotropin receptor p75NTR | Polyclonal rabbit | Promega Corp.,Madison, WI | 1:500 |
*- these two antibodies gave similar results.