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. 2015 Jan 26;4:e04105. doi: 10.7554/eLife.04105
Reagent Type Manufacturer Catalog # Comments
pPGK-SB13 transposase vector DNA Vector N/A N/A Original reagent obtained from authors
pT/CaggsNrasG12V transposon vector DNA Vector N/A N/A Original reagent obtained from authors
pT/CaggsNrasG12V/D38A transposon vector DNA Vector N/A N/A Original reagent obtained from authors
Ketamine HCl Anesthetic Specific brand information will be left up to the discretion of the provider lab and recorded later
Acepromazine maleate Anesthetic
Butorphanol tartrate Anesthetic
Dulbecco's Phosphate Buffered Saline Reagent Sigma–Aldrich D1408 Original brand not specified
Paraformaldehyde Reagent Sigma–Aldrich 158127 Original brand not specified
C57/BL6J (H-2b) Mouse line Jackson Laboratory Strain #000664
C57/BL6 CD4−/− (H-2b) (B6.129S2-CD4tm1Mak/J) Mouse line Jackson Laboratory Strain #002663
Mouse anti-Nras (F155) Antibody Santa Cruz Sc-31
Biotinylated goat anti-mouse IgG1-conjugate Antibody Thermo-Fisher TM-060-BN
IgG1 mouse isotype control antibody Antibody Sigma–Aldrich M5284
Diaminobenzidine (DAB) IHC Stain Specific brand information will be left up to the discretion of the provider lab and recorded later
Haematoxylin IHC Stain
Eosin IHC Stain