Table 4.
Sequences of endogenous TCR beta chains of insulin 2 B:9-23 peptide-responsive hybridomas generated from BDC 12-4.1 TCR α+β−Cα−/− transgenic mice or BDC12-4.4 TCR α+Cα−/−NOD/SCID retrogenic mice.
| Source mice | V beta (CDR3) | n region | J beta |
|---|---|---|---|
|
| |||
| BDC12-4.1 alpha chain transgenic mice | TRBV3: | TRBJ2-3*01: | |
| VERPDGSYFTLKIQPTALEDSAVYFCASSL | DGGVC | AETLYFGSGTRLTVL | |
|
| |||
| TRBV13-3: | TRBJ2-7*01: | ||
| KASRPSQENFSLILELASLSQTAVYFCASSD | APF | YEQYFGPGTRLTVL | |
| TRBV13-3: | TRBJ2-5*01: | ||
| KASRPSQENFSLILELASLSQTAVYFCASS | WDWGGVE | DTQYFGPGTRLLVL | |
|
| |||
| TRBV15: | TRBJ1-1*01: | ||
| AEMLNSSFSTLKIQPTEPKDSAVYLCASSL | RP | NTEVFFGKGTRLTVV | |
|
| |||
| TRBV16: | TRBJ1-1*01: | ||
| AQMPNQSHSTLKIQSTQPQDSAVYLCASSL | DRGQA | NTEVFFGKGTRLTVV | |
|
| |||
| TRBV19: | TRBJ1-1*01: | ||
| ASREKKSSFSLTVTSAQKNEMAVFLCASSI | RG | TEVFFGKGTRLTW | |
| TRBV19: | TRBJ2-4*01: | ||
| ASREKKSSFSLTVTSAQKNEMAVFLCASSI | WDWG | NTLYFGAGTRLSVL | |
|
| |||
| BDC12-4.4 alpha chain retrogenic mice | TRBV1: | TRBJ1-5*02: | |
| ATRVTDTELRLQVANMSQGRTLYCTCSA | VGC | QPAPLFGEGTRLSVL | |
|
| |||
| TRBV5: | TRBJ2-2*01: | ||
| PECPDSSKLLLHISAVDPEDSAVYFCASSQ | ET | NTGQLYFGEGSKLTVL | |
| TRBV5: | TRBJ2-5*01: | ||
| PECPDSSKLLLHISAVDPEDSAVYFCASSQ | ETGGGG | DTQYFGPGTRLLV | |