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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Virology. 2015 Feb 25;478:50–60. doi: 10.1016/j.virol.2015.02.002

Figure 2. Chemical inhibition of Notch1 abrogated canonical pathway components.

Figure 2

(A) Representative immunoblot of cleaved Notch1 protein in 16E6 HFKs transduced with overexpressed FLAG-tagged NFX1-123 wild type (FNFX1-123WT) or LXSN vector control. Whole cell extracts from 16E6/LXSN and 16E6/FNFX1-123WT treated with either DMSO or 10µM DAPT were prepared at the indicated times. Actin was used as a loading control. Similar results were obtained in two additional experiments. (B) Relative levels of Hes1 mRNA (C) Hes5 mRNA, and (D) Notch1 mRNA were calculated using the ΔΔCT method, normalizing mRNA levels to GAPDH within each sample. Values shown were the mean fold change in each sample compared to the DMSO treated 16E6/LXSN vector control. Error bars represent the standard deviation for triplicate samples.