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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Virology. 2015 Feb 25;478:50–60. doi: 10.1016/j.virol.2015.02.002

Figure 5. Differentiation markers unperturbed by chemical inhibition of Notch1 in 16E6 HFKs with overexpressed NFX1-123.

Figure 5

16E6 HFKs transduced with overexpressed FLAG-tagged NFX1-123 wild type (FNFX1-123WT) or LXSN vector control treated with either DMSO or 10µM DAPT were prepared at the indicated times. Similar results were obtained in three independent experiments. (A) Relative levels of Keratin 1 mRNA and (B) Keratin 10 mRNA were calculated using the ΔΔCT method, normalizing mRNA levels to GAPDH within each sample. Values shown were the mean fold change in each sample compared to the DMSO treated 16E6/LXSN vector control. Error bars represent the standard deviation for triplicate samples. (C) Transcript levels for p21Waf1/CIP1 were assessed by quantitative real-time PCR using primers and normalized to the housekeeping gene 36B4 as control. Values shown were the mean fold change in each sample compared to the DMSO treated 16E6/LXSN vector control. Error bars represent 95% confidence intervals from the technical triplicate replicates shown.