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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Virology. 2015 Feb 25;478:50–60. doi: 10.1016/j.virol.2015.02.002

Figure 6. Notch1 stimulation by DL1 activated differentiation markers in 16E6 HFKs with overexpressed NFX1-123.

Figure 6

16E6/LXSN and 16E6/FNFX1-123WT HFKs were plated on dishes coated with purified Delta-1 ligand (DL1) immobilized on retronectin or retronectin alone. Whole cell extracts were harvested for RNA and protein at the indicated times. Similar results were found in at least three independent experiments. (A) Relative levels of Keratin 1 mRNA and (B) Keratin 10 mRNA were calculated using the ΔΔCT method, normalizing mRNA levels to GAPDH within each sample. Values shown were the mean fold change in each sample compared to the 16E6/LXSN vector control plated on retronectin. Error bars represent the standard deviation for each triplicate. (C) Transcript levels for p21Waf1/CIP1 were assessed by quantitative real-time PCR using primers and normalized to the housekeeping gene 36B4 as control. Values shown were the mean fold change in each sample compared to the DMSO treated 16E6/LXSN vector control. Error bars represent 95% confidence intervals from the technical triplicate replicates shown.