MT1A2 |
Cell line |
Original lab |
n/a |
From original lab |
Dulbecco's Modified Eagle's Medium, high glucose with HEPES
modification |
Cell culture |
Sigma–Aldrich |
D6171 |
Included during communication with original authors.
Original lab used Gibco catalog # 12430-054 |
L-glutamine |
Cell culture |
Sigma–Aldrich |
G7513 |
Fetal bovine serum |
Cell culture |
Sigma–Aldrich |
F0392 |
|
Penicillin/Streptomycin (100×) |
Cell culture |
Sigma–Aldrich |
P433 |
|
Trypsin-EDTA |
Cell culture |
Sigma–Aldrich |
T3924 |
|
Hank's balanced salt solution |
Cell culture |
Sigma–Aldrich |
H6648 |
|
T75 flask |
Labware |
Sigma–Aldrich |
Z707503 |
|
50-ml tubes |
Labware |
Sigma–Aldrich |
CLS430290 |
|
1 M Hepes in normal saline |
Buffer |
Biowhittaker |
17-737E |
Included during communication with original authors |
BSA, IgG free |
Chemical |
Jackson Immunoresearch |
001-000-161 |
Included during communication with original authors. |
Kolliphor P188 |
Chemical |
Sigma–Aldrich |
K4894 |
Included during communication with original authors. Original lab
used Pluronic F-68 which has been discontinued |
Leibovitz L15 media, no phenol red |
Cell culture |
Life Technologies |
21083027 |
Included during communication with original authors |
Matrigel Matrix High Concentration* |
Cell culture |
Corning |
354248 |
Original from Becton Dickinson |
6–8 week old female FVB mice |
Animal model |
Charles River |
Strain Code: 207 |
Original from Jackson Labs |
27½G needle |
Labware |
Sigma–Aldrich |
Z192384 |
Included during communication with original authors |
1-ml syringe |
Labware |
Sigma–Aldrich |
Z192090 |
|
30½G needle |
Labware |
Sigma–Aldrich |
Z192341 |
Included during communication with original authors. Original lab
used 31 gauge |
1–5% isoflurane |
Chemical |
Specific brand information will be left up to the
discretion of the replicating lab and recorded later |
Anti-CD47 clone MIAP410 |
Antibody |
Original lab |
n/a |
From original lab |
Mouse IgG isotype control |
Antibody |
Innovative Research |
IR-MS-GF |
|
PBS |
Buffer |
Sigma–Aldrich |
D8537 |
|
Hematology analyzer* |
Instrument |
Idexx Laboratories |
ProCyte Dx |
Original lab used a Heska HemaTrue |
Neutral buffered formalin |
Buffer |
Specific brand information will be left up to
the discretion of the replicating lab and recorded later |
Ethanol |
Chemical |
Xylene |
Chemical |
Paraffin |
Chemical |
Carazzi's Hematoxylin |
Stain |
Eosin |
Stain |
Permount |
Chemical |