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. 2015 Jan 26;4:e04586. doi: 10.7554/eLife.04586
Reagent Type Manufacturer Catalog # Comments
MT1A2 Cell line Original lab n/a From original lab
Dulbecco's Modified Eagle's Medium, high glucose with HEPES modification Cell culture Sigma–Aldrich D6171 Included during communication with original authors. Original lab used Gibco catalog # 12430-054
L-glutamine Cell culture Sigma–Aldrich G7513
Fetal bovine serum Cell culture Sigma–Aldrich F0392
Penicillin/Streptomycin (100×) Cell culture Sigma–Aldrich P433
Trypsin-EDTA Cell culture Sigma–Aldrich T3924
Hank's balanced salt solution Cell culture Sigma–Aldrich H6648
T75 flask Labware Sigma–Aldrich Z707503
50-ml tubes Labware Sigma–Aldrich CLS430290
1 M Hepes in normal saline Buffer Biowhittaker 17-737E Included during communication with original authors
BSA, IgG free Chemical Jackson Immunoresearch 001-000-161 Included during communication with original authors.
Kolliphor P188 Chemical Sigma–Aldrich K4894 Included during communication with original authors. Original lab used Pluronic F-68 which has been discontinued
Leibovitz L15 media, no phenol red Cell culture Life Technologies 21083027 Included during communication with original authors
Matrigel Matrix High Concentration* Cell culture Corning 354248 Original from Becton Dickinson
6–8 week old female FVB mice Animal model Charles River Strain Code: 207 Original from Jackson Labs
27½G needle Labware Sigma–Aldrich Z192384 Included during communication with original authors
1-ml syringe Labware Sigma–Aldrich Z192090
30½G needle Labware Sigma–Aldrich Z192341 Included during communication with original authors. Original lab used 31 gauge
1–5% isoflurane Chemical Specific brand information will be left up to the discretion of the replicating lab and recorded later
Anti-CD47 clone MIAP410 Antibody Original lab n/a From original lab
Mouse IgG isotype control Antibody Innovative Research IR-MS-GF
PBS Buffer Sigma–Aldrich D8537
Hematology analyzer* Instrument Idexx Laboratories ProCyte Dx Original lab used a Heska HemaTrue
Neutral buffered formalin Buffer Specific brand information will be left up to the discretion of the replicating lab and recorded later
Ethanol Chemical
Xylene Chemical
Paraffin Chemical
Carazzi's Hematoxylin Stain
Eosin Stain
Permount Chemical