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. 2015 Mar 19;2015:468457. doi: 10.1155/2015/468457

Figure 2.

Figure 2

Effects of knockdown of RNA sensors and their adaptor molecules on reovirus-induced expression of IFN-β and Noxa in tumor cells. (a) Knockdown efficiencies of siRNAs. Cells were transfected with siRNAs targeting indicated gene or control siRNA (siControl). Following a 48-hour incubation, cells were harvested for the evaluation of siRNA-mediated knockdown efficiencies by qRT-PCR analysis. (b) mRNA levels of IFN-β and Noxa following reovirus infection in the cells pretreated with siRNAs. Forty-eight hours after transfection with siRNAs, cells were infected with reovirus at an MOI of 20. Cells were harvested 24 hrs after infection for analysis of IFN-β and Noxa mRNA levels by qRT-PCR analysis. The data were normalized by the data of the siControl group infected with reovirus. The data shown represent the mean ± SD of triplicate measurements. * P < 0.05, ** P < 0.01, and *** P < 0.001, compared with the siControl group infected with reovirus.