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. 2015 Apr 2;10(4):e0122411. doi: 10.1371/journal.pone.0122411

Fig 3. Analysis of the functionality of the exogenous TcdR in two clostridial strains (CRG3011 and CRG3817).

Fig 3

Plasmid maps of the pMTL82254-PtcdB (A) and pMTL82254-Pfdx (B). Key: CD0164 terminator, a transcriptional terminator isolated from downstream of the C. difficile strain 630 CD0164 gene; catP, a C. perfringens-derived gene encoding chloramphenicol acetyltransferase; Cpa fdx terminator, transcriptional terminator of the ferredoxin gene of C. pasteurianum; repA and orf2, replication region of the C. botulinum plasmid pBP1; ermB, the macrolide-lincosamide-streptogramin B antibiotic resistance gene of plasmid pAMß1; ColE1, the replication origin of plasmid ColE1, and; traJ, transfer function of the RP4 oriT region. CD tcdB promoter, the promoter region of the C. difficile tcdB gene; Csp fdx promoter: the promoter region of the C. sporogenes fdx gene. (C): CAT activity of either C. acetobutylicum ATCC 824 wild type or CRG3011 (tcdR containing C. acetobutylicum ATCC 824) carrying plasmids pMTL82254-PtcdB and pMTL82254-Pfdx. (D): CAT activity of either C. sporogenes NCIMB 10969 wild type or CRG3817 (tcdR containing C. sporogenes NCIMB 10969) carrying plasmids pMTL82254-PtcdB and pMTL82254-Pfdx. Black circles ●, wild type with pMTL82254-PtcdB; black squares ■, wild type with pMTL82254-Pfdx; black triangles ▲, CRG3011/CRG3817 with pMTL82254-PtcdB; black triangles ▼, CRG3011/CRG3817 with pMTL82254-Pfdx.