Table 1. Bacterial strains and plasmids used in this study.
| Strain or Plasmid | Relevant characteristics a | Source b |
|---|---|---|
| Bacterial Strains | ||
| Clostridium acetobutylicum ATCC 824 | Wild type | ATCC |
| Clostridium sporogenes NCIMB 10696 | Wild type | NCIMB |
| E. coli TOP10 | Invitrogen | |
| CRG3011 | C. acetobutylicum ATCC 824 with tcdR inserted at pyrE locus | This study |
| CRG3817 | C. sporogenes NCIMB 10696 with tcdR inserted at pyrE locus | This study |
| Plasmids | ||
| pMTL83251 | Clostridium modular plasmid used for construction of IPTG inducible promoter system. EmR | [32] |
| pMTL-ME6C | ACE vector for DNA integration at pyrE locus in C. acetobutylicum 824. CmR | [10] |
| pMTL-YZ29 | ACE vector for DNA integration at pyrE locus in C. sporogenes NCIMB 10696. CmR | This study |
| pMTL-ME6C-tcdR | ACE plasmid for tcdR integration at pyrE locus in C. acetobutylicum 824. CmR | This study |
| pMTL-YZ29-tcdR | ACE plasmid for tcdR integration at pyrE locus in C. sporogenes NCIMB 10696. CmR | This study |
| pMTL83251-lacI | Clostridium modular plasmid with conditional replicon | This study |
| pMTL83251-lacI-T | Clostridium modular plasmid with conditional replicon and a fdx terminator | This study |
| pMTL87250 | Clostridium modular plasmid with conditional replicon | This study |
| pMTL-YZ13 | Transposon plasmid with pCB102 replicon | This study |
| pMTL-YZ14 | Transposon plasmid with conditional replicon | This study |
| pMTL82254-PtcdB | Clostridium modular plasmid with catP reporter expressed by promoter tcdB | This study |
| pMTL82254-Pfdx | Clostridium modular plasmid with catP reporter expressed by promoter fdx | This study |
a CmR, chloramphenicol/thiamphenicol resistance gene; EmR, erythromycin resistance gene
b ATCC, American Type Culture Collection; NCIMB, The UK's National Collection of Industrial, Food and Marine Bacteria