|
Sequence (5’-3’) |
Description |
| Del-traJ |
AAAAAAGCTTATAATTATCCTTATTGGACTTTCAAGTGCGCCCAGATAGGGTG |
Delete traJ in pMTL5401Fcat |
| Del-traJ-antisense |
CAGATTGTACAAATGTGGTGATAACAGATAAGTCTTTCAATTTAACTTACCTTTCTTTGT |
Delete traJ in pMTL5401Fcat |
| YZ4 |
TGAACGCAAGTTTCTAATTTCGATTTCCAATCGATAGAGGAAAGTGTCT |
Amplify IPTG cassette |
| YZ35 |
GCTGATATGGTAATGAAGGG |
Amplify IPTG cassette |
| NotI/AscI |
AACAGCTATGACCGGCGCGCCGCTCACTGCCCGC |
Change NotI site to AscI in pMTL83251-lacI |
| NotI/AscI-antisense |
GCGGGCAGTGAGCGGCGCGCCGGTCATAGCTGTT |
Change NotI site to AscI in pMTL83251-lacI |
| tcdR-F |
GCTATATCAAGTGCTAAAGGTC |
Amplify tcdR gene |
| tcdR-R |
AAAAAAGCTTATAATTATCCTTAGCGGTCCAAGACGTGCGCCCAGATAGGGTG |
Amplify tcdR gene |
| catP-INV-F1 |
CAGATTGTACAAATGTGGTGATAACAGATAAGTCCAAGACTCTAACTTACCTTTCTTTGT |
Inverse PCR [15] |
| catP-INV-R1 |
TGAACGCAAGTTTCTAATTTCGATTACCGCTCGATAGAGGAAAGTGTCT |
Inverse PCR[15] |
| catP-INV-R2 |
GGATCTGTAATAACCCATAAAG |
Sequencing of inverse PCR products[15] |
