Table 4. Characterization of the found auxotroph mutant with different additives in P2 minimal medium.
Supplementation | citB (CAC0972) mutant | C acetobutylicum CRG3011 |
---|---|---|
Citrate | ˗˗ | ++ |
Iso-citrate | ˗˗ | ++ |
α-Ketoglutarate | ˗˗ | ++ |
Glutamate | ++ | ++ |
Glutamine | + | ++ |
Proline | ˗˗ | ++ |
Arginine | ˗˗ | ++ |
Without supplementation | ˗˗ | ++ |
No growth(˗˗), slight growth(+) and normal growth(++).