Table 2.

Effect of various treatments on intracellular water content of liver slices in the presence and absence of ouabain.

Intracellular water content (kg water/kg dry wt)a
Incubationb
Treatment	90 min at 1°C	Then, at 38°C for 60 min
		Control	Treatment	Ouabain	Ouabain plus treatment	(n)
Cytochalasin B (100 μg/mL)	2.42 ± 0.33	1.48 ± 0.10	1.60 ± 0.17	1.72 ± 0.10	2.19 ± 0.11c	8
Cytochalasin E (33 μg/mL)	2.77 ± 0.07	1.75 ± 0.13	1.92 ± 0.08	1.96 ± 0.06	2.38 ± 0.13c,d	18
Colchicine (1 mM)	2.73 ± 0.10	1.68 ± 0.11	1.61 ± 0.08	1.98 ± 0.17	2.15 ± 0.25	10
Cl− free medium, with NO3 − as substitute	2.60 ± 0.09	1.52 ± 0.08	1.70 ± 0.08	2.00 ± 0.06	2.47 ± 0.13c	15

  ^aIntracellular water was determined from total tissue water, measured gravimetrically, and the volume of distribution of inulin [9]. Values are the mean ± standard error of the mean.

^bSlices were incubated in a medium containing 146 mM Na⁺, 5 mM K⁺, 1 mM Mg²⁺, 1.3 mM Ca²⁺, 161 mM Cl⁻, 2 mM phosphate, 1 mM SO₄ ²⁻, and Tris (10 mM pH 7.4). It was gassed with O₂. Cl-free medium contained NO₃ ⁻ instead of Cl⁻. Ouabain was at 2 mM. Cytochalasins were added from stock solutions in dimethyl sulfoxide; final concentration in medium was 0.1% (v/v) DMSO.

^cSignificantly greater than value with ouabain alone, by t-test; P < 0.01.

^dSignificantly less than value after incubation at 1°C, by t-test; P < 0.01.