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. 2015 Apr 2;10(4):e0122442. doi: 10.1371/journal.pone.0122442

Fig 4. GCs enhanced cell-cell aggregation and reduced the motility and invasiveness of kidney cancer cells.

Fig 4

(A) Phase contrast images of a cell-cell aggregation assay showing the ability of GCs to enhance cohesion of cells. The graph represents the mean of three independent experiments performed in quadruplicate wells ±SE. (B) The distance migrated by cells pre-treated with DMSO or GCs in a wound healing assay after 24 hr. The data represent the mean±SE of three independent experiments. Asterisk indicates p-value <0.05 by Student’s t-test comparing DMSO versus drug treatment. (C) The reduction of Caki-1 migration by drug treatment was not a result of changes in cell proliferation as shown by an MTT assay. This was performed in three independent experiments with 12 wells per condition. The graph is from a single representative experiment with bars representing the mean±SE. (D) A Transwell invasion assay showing the invasive capacity of Caki-1 cells through collagen coated inserts at 24 hr. Data represent means±SE of three independent experiments performed in duplicate wells. Asterisk indicates significant p-value < 0.05 by Student’s t-test.