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. 2015 Apr 2;10(4):e0121703. doi: 10.1371/journal.pone.0121703

Fig 5. Protective effect of 9-Phe against hypoxia-reperfusion-induced death in H9c2 cardiomyocytes.

Fig 5

Cultures of H9c2 cells, incubated with DMSO or 9-Phe (10 or 20 μM), were subjected to hypoxia-reperfusion (H/R; 4 h anoxia followed by 1 h reoxygenation). Viability was measured by the MTT assay, and absorbance was normalized to that of the normoxic condition. The presence of 9-Phe (≥10 μM) completely prevented the damage caused by H/R (n = 3 for each condition). Dunnett’s post hoc test was performed. * p < 0.05, N.S.: p > 0.05.