Fig 1. SGK3 expression in relation to (activated) signalling components of the PI3K/Akt system in MM cell lines.

Shown are Western blots for PI3K pathway-associated signalling proteins or for their phosphorylated forms. Cells from the MM cell lines indicated were harvested from standard cell culture, the signals are thus representative of steady-state levels in culture. One cell lysate per line was used to load multiple gels. The representative β-actin control derives from the same blot on which SGK3 and P-FOXO1/3A were also stained. Note: the strong phospho-STAT3 signal in INA-6 cells results from permanent supplementation of the culture with recombinant human IL-6.