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. 2014 Nov 21;123(4):324–330. doi: 10.1289/ehp.1408626

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Decay rates of four probe set–identified transcripts for which the steady-state levels and decay rates were significantly different in arsenite-treated cells compared with control. Cells were treated with vehicle or arsenite for 24 hr and then treated with actinomycin D. Probe set–identified transcript levels were measured by microarray before and at 1 hr intervals after actinomycin D treatment. The starting levels of each probe set–identified transcript after 24 hr of treatment but before actinomycin D were set at 100%, and the data are expressed as mean percentages (± SD) of the average starting value (n = 4 biological replicates per group). In all cases, the decay curves were significantly different between control and arsenite-treated cells by both the ORIOGEN and EDGE methods (p < 0.05). The Affymetrix probe set identifiers for the transcripts shown are as follows: ALAS1, 205633_s_at; GATA3, 209602_s_at; MAP3K8, 205027_s_at; and KRTAP1‑3, 234880_x_at.