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. 2015 Apr 2;11(4):e1004801. doi: 10.1371/journal.ppat.1004801

Fig 5. Iug6, Iug9, Nup1, Nup2, and Nup3 suppress the cell death triggered by BAX.

Fig 5

(A) Agroinfiltration sites in N. benthamiana leaves expressing Iug6, Iug9, Nup1, Nup2, or Nup3 were challenged with A. tumefaciens expressing the BAX elicitin. The BAX-induced cell death was scored at 3 and 4 DAI. A. tumefaciens strain carrying pGR106-GFP was used as a negative control, and pGR106-BAX as a positive control. (B) Western blot analysis of GFP, Iug6, Iug9, Nup1, Nup2, Nup3, and Bax protein levels in plant tissues treated above. Proteins were extracted 60 h after the last infiltration. Equal amounts of protein lysate were loaded in each lane, as verified by Ponceau S staining. (C) Presence or absence polymorphisms of each candidate effectors are indicated by a colored or black tile across the 29 field isolates. Colored tile, presence; black tile, absence. Presence /absence patterns (top) and isolates (left) were hierarchically clustered.